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KMID : 0364219910340010031
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Korean Journal of Zoology
1991 Volume.34 No. 1 p.31 ~ p.43
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Immunogold Electron Microscopy on Skeletal Myofibrilogenesis of Chick Embryo
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Hah Jae-Chung
Kim Jeong-Ok
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Abstract
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In the present study, the localization of myosin and the pathway of myofibrilogenesis of skeletal muscle cells in culture were investigated by immunocytochemical methods including immunoelectron microscopy and hybridoma formation. There were manly free ribosomes in the cytosol of the myoblast. At 48 hr of the culture, the myofilaments started to form and at 72 hr began to assemble to form myoabrils. At this time the Z band appeared, but the sarcomeres did not link together. Aker 96 hr of culture, adjacent sarcomeres linked together and shotved the typical striation of the skeletal muscle fiber. Myosin was synthesized in free ribosomes at 24 hr of culture and localized at many myofilaments at 48 hr and assembled mvoabrils at 72 hr and at 120 hr of the culture, myosin was localized at thick filaments of the A band.
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KEYWORD
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Myosin synthetic pathway, Myofibrilogenesis, Immunogold electron microscopy
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